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2018, v.40;No.161(03) 277-284

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10种帘蛤科贝类COI基因序列分析及系统发育研究
Study on mitochondrial COI gene sequences and molecular phylogeny of ten Veneridae species

陈道海;李洪英;吴秋颖;孙玉林;文菁;
CHEN Daohai;LI Hongying;WU Qiuying;SUN Yulin;WEN Jing;Round Beibu Gulf Institute for the Protection and Utilization of Marine Animals in Medicine,Lingnan Normal University;Life Science and Technology School,Lingnan Normal University;

摘要(Abstract):

应用通用引物扩增了凸加夫蛤(Gafrarium tumidum)、锯齿巴非蛤(Paphia gallus)、细纹卵蛤(Pitar striatum)、钝缀锦蛤(Tapes dorsatus)、裂纹格特蛤(Marcia hiantina)5种帘蛤科贝类COI基因片段,并与Gen Bank数据库收录的加夫蛤(Gafrarium pectinatum)、沟纹巴非蛤(Paphia exarata)、日本卵蛤(Pitar japonicum)、日本格特蛤(Marcia japonica)、四射缀锦蛤(Tapes belcheri)5种帘蛤科贝类的同源序列进行比对分析。结果表明:所有物种扩增片段长度均为616 bp,序列A+T平均含量(62.9%)明显高于G+C含量。在616个位点中,保守位点数为282个,变异位点数为334个,其中简约信息位点数为283个。以COI基因片段序列为标记,以海螂科砂海螂(Mya arenaria)作外群,构建了帘蛤科贝类的系统进化树,其拓扑结构显示:细纹卵蛤和日本卵蛤聚为一枝,凸加夫蛤和加夫蛤聚为一枝,锯齿巴非蛤和沟纹巴非蛤聚为一枝,四射缀锦蛤单独聚为一枝,钝缀锦蛤、裂纹格特蛤和日本格特蛤聚为一枝,最后所有帘蛤科物种聚为一枝,与外群相区别,其结果与传统形态分类基本一致。研究表明,线粒体COI基因作为帘蛤科贝类DNA条形码在物种鉴定方面具有可靠性,可以作为物种分类的重要辅助手段。
The COI genes(a 616 base pair region)of five Veneridae species(Gafrarium tumidum,Paphia gallus,Pitar striatum,Tapes dorsatus,and Marcia hiantina)were amplified using universal primers and sequenced,respectively. Subsequently,they were compared with those of the other five selected Veneridae species(Gafrarium pectinatum,Paphia exarata,Pitar japonicum,Marcia japonica,and Tapes belcheri). The result indicated that the mean content of A+T was 62.9% and higher than that of G+C. The 616 bp COI gene sequence had 282 conservative sites and 334 variable sites,the latter included 283 parsimony informative sites. A phylogenetic tree of 10 Veneridae species was reconstructed by using the COI gene sequences as marker,and with Mya arenaria as the outgroup. The tree topologies indicated that Gafrarium tumidum with its sister taxon Gafrarium pectinatum,Paphia gallus with Paphia exarata,Pitar striatum with Pitar japonicum,Tapes dorsatu,Marcia hiantina with Marcia japonica,and Tapes belcheri alone formed a monophyletic group,respectively. Moreover,all Veneridae species could form a clade that differentiated from the outgroup species,which was consistent with the tree based on morphological characters. Therefore,COI-based DNA barcoding of Veneridae provides a basis data for the applicability of species identification and a necessary supplement for taxonomy.

关键词(KeyWords): 帘蛤科;线粒体COI基因;系统发育
Veneridae;mitochondrial COI gene;phylogeny

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基金项目(Foundation): 广东省科技厅项目(2014B040404071);; 湛江市财政资金科技专项竞争性分配项目(2015A06008)

作者(Author): 陈道海;李洪英;吴秋颖;孙玉林;文菁;
CHEN Daohai;LI Hongying;WU Qiuying;SUN Yulin;WEN Jing;Round Beibu Gulf Institute for the Protection and Utilization of Marine Animals in Medicine,Lingnan Normal University;Life Science and Technology School,Lingnan Normal University;

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