生物资源

2018, v.40;No.161(03) 218-225

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响应面法优化火龙果茎甾醇的提取及纯化工艺研究
Optimization of extraction and purification of phytosterol from pitaya stem by response surface methodology

欧岳姣;曹剑锋;张美容;陈集双;梁西琴;胡灿;罗朝琴;徐连巧;
OU Yuejiao;CAO Jianfeng;ZHANG Meirong;CHEN Jishuang;LIANG Xiqin;HU Can;LUO Chaoqin;XU Lianqiao;School of Chemistry and Life Science,Guizhou Education University;Guizhou Institute of Medicinal Plant Biotechnology,Guizhou Education University;Guizhou Characteristic Key Laboratory of Biological Resource Exploitation and Utilization,Guizhou Education University;College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University;

摘要(Abstract):

以火龙果茎为原料,研究火龙果茎甾醇的提取及精制工艺。利用响应面分析法对火龙果茎甾醇超声提取工艺进行优化。结果表明:超声波辅助提取火龙果茎甾醇的最佳工艺条件为提取时间57 min、超声功率143 W、液料比12∶1(m L/g)、温度53℃,甾醇得率为2.693‰。通过正交试验确定火龙果茎甾醇精制的最佳条件为:液料比为4∶1(m L/mg),结晶初始温度为55℃,养晶时间为18 h,此条件下火龙果茎甾醇的纯度达到87.6%。
In this study,the extraction and purification technologies of phytosterol from pitaya stem were investigated. The response surface methodology was used to optimize ultrasound-assisted extraction of phytosterol from pitaya stem. The result showed that the best ultrasonic extraction conditions were as follows:the ultrasonic time was 57 min,the ultrasonic power was 143 W,the ultrasonic temperature was 53℃,and the liquid to solid ratio was 12∶1(m L/g).The total phytosterol extraction yield was 2.693‰. The optimum purification conditions of phytosterol from pitaya stem were determined by the orthogonal experiment:the liquid to solid ratio was 4∶1(m L/mg),initial crystallization temperature was 55 ℃,and crystallization time was 18 h. The purity of phytosterol from pitaya stem reached up to 87.6% under the optimum conditions.

关键词(KeyWords): 火龙果茎;植物甾醇;响应面;纯化
pitaya stem;phytosterol;response surface;purification

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基金项目(Foundation): 贵州省教育厅自然基金项目(黔教合KY字[2015]374);; 贵州省科技厅自然科学基金重点项目(黔科合基础[2016]1415);; 贵州省大学生创新训练项目黔教高发[2016]201号;; 贵州省一流师资团队建设项目(黔高教发[2017]158号);; 贵州省茶籽资源综合开发利用工程研究中心项目[黔教合KY字[2017]020]

作者(Author): 欧岳姣;曹剑锋;张美容;陈集双;梁西琴;胡灿;罗朝琴;徐连巧;
OU Yuejiao;CAO Jianfeng;ZHANG Meirong;CHEN Jishuang;LIANG Xiqin;HU Can;LUO Chaoqin;XU Lianqiao;School of Chemistry and Life Science,Guizhou Education University;Guizhou Institute of Medicinal Plant Biotechnology,Guizhou Education University;Guizhou Characteristic Key Laboratory of Biological Resource Exploitation and Utilization,Guizhou Education University;College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University;

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