范沾涛,韦范,乔柱,梁莹,谢唐贵,刘吉华,韦坤华
FAN Zhantao,WEI Fan,QIAO Zhu,LIANG Ying,XIE Tanggui,LIU Jihua,WEI Kunhua

• 1:广西药用植物园广西药用资源保护与遗传改良重点实验室
• 2:广西药用植物园广西壮族自治区中药资源智慧创制工程研究中心
• 3:中国药科大学江苏省中药评价与转化重点实验室

摘要(Abstract)：

次生代谢产物是药用植物的重要有效成分，但由于野生资源日益减少和栽培品种品质退化，给临床使用和质量控制带来许多困扰，利用组织培养技术生产有效成分，能缓解药用植物资源压力。本文总结了在植物组织培养过程中，外植体的选择、培养条件、培养方法等因素对次生代谢产物积累的影响，并简述了应用诱导子、添加前体物、添加抑制剂和应用基因工程技术四种促进次生代谢产物积累的方法，旨在为利用组织培养技术生产次生代谢产物提供理论参考，为药用植物资源的开发和利用提供科学指导。
Many factors are involved in the production of secondary metabolites by tissue culture of medicinal plants.In this paper,the influences of the selection of explants,culture conditions and culture methods on the production of secondary metabolites are summarized,and four methods to improve the production of secondary metabolites,including the application of inducers,the addition of precursors,the addition of inhibitors and the application of genetic engineering technology,are described.

关键词(KeyWords)： 药用植物;组织培养;次生代谢产物
medicinal plant;tissue culture;secondary metabolite

Abstract:

Keywords:

基金项目(Foundation): 中央引导地方科技发展专项资金项目（桂科ZY20198018）;; 广西创新驱动发展专项（桂科AA18242040）;; 对发展中国家科技援助项目（KY201904001）;; “广西八桂学者”专项经费项目;; 广西科研创新团队建设项目（桂药创201905）

作者(Author): 范沾涛,韦范,乔柱,梁莹,谢唐贵,刘吉华,韦坤华
FAN Zhantao,WEI Fan,QIAO Zhu,LIANG Ying,XIE Tanggui,LIU Jihua,WEI Kunhua

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